Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium

In Belgium, the incorporation of phages into magistral preparations for human application has been permitted since 2018. The stability such is high importance to guarantee quality and efficacy throughout treatments. We evaluated ability preserve infectivity four different active against three bacterial species in five buffer infusion solutions commonly used medicine biotechnological manufacturing processes, at two concentrations (9 7 log pfu/mL), stored 4 °C. DPBS without Ca2+ Mg2+ was found be best option, compared other solutions. Suspensions with phage pfu/mL were unsuited as their activity dropped below effective therapeutic dose (6–9 even after one week storage Strong variability between observed, Acinetobacter baumannii Acibel004 being stable out also studied long term lyophilized staphylococcal ISP, that titer could preserved during a period almost 8 years when sucrose trehalose stabilizers. After rehydration ISP saline, remained °C 126 days.